Abundance and biomass of phytoplankton in the western part of the Black Sea during Branimir Ormanov cruise BO092000 in September 2000

The samples were concentrated down to 50 cm**3 by slow decantation after storage for 20 days in a cool and dark place. The species identification was done under light microscope OLIMPUS-BS41 connected to a video-interactive image analysis system at magnification of the ocular 10X and objective - 40X. A Sedgwick-Rafter camera (1ml) was used for counting. 400 specimen were counted for each sample, while rare and large species were checked in the whole sample (Manual of phytoplankton, 2005). Species identification was mainly after Carmelo T. (1997) and Fukuyo, Y. (2000).
Taxon-specific phytoplankton abundance and biomass were analysed by Moncheva S., B. Parr, 2005. Manual for Phytoplankton Sampling and Analysis in the Black Sea.
The cell biovolume was determined based on morpho-metric measurement of phytoplankton units and the corresponding geometric shapes as described in detail in (Edier, 1979).

将样品置于阴凉避光处储存20日后，通过缓慢倾析法浓缩至50立方厘米（cm³）。物种鉴定通过连接至视频交互式图像分析系统的OLIMPUS-BS41光学显微镜完成，目镜放大倍数为10×，物镜放大倍数为40×。采用容积为1毫升的赛奇-拉夫特计数池（Sedgwick-Rafter camera）进行计数，每个样品计数400个个体，对于稀有及大型物种则对全样进行检查（《浮游植物手册》，2005）。物种鉴定主要参考Carmelo T.（1997）与Fukuyo, Y.（2000）的研究成果。
类群特异性浮游植物（phytoplankton）丰度与生物量的分析参照Moncheva S.、B. Parr于2005年编著的《黑海浮游植物采样与分析手册》。
细胞生物体积的测定基于浮游植物单元的形态计量学测量结果及其对应的几何形态，详细方法参见Edier（1979）的相关研究。