Deep mutational scanning of SARS-related coronavirus RBDs using yeast display of barcoded libraries

We performed deep mutational scanning and related experiments on a collection of wildtype and mutated receptor-binding domains (RBDs) from a panel of SARS-related coronavirus isolates, including a pan-sarbecovirus survey library and complete deep mutational scans in RshSTT182, SARS-CoV-1, RsYN04, PRD-0038, and RmYN02. We used these libraries to measure phenotypes such as binding to ACE2 orthologs or antibodies.

我们针对一组源自多株SARS相关冠状病毒分离株的野生型与突变型受体结合域（receptor-binding domains, RBD）开展了深度突变扫描（deep mutational scanning）及相关实验，实验涵盖泛沙贝病毒筛选文库（pan-sarbecovirus survey library），以及针对RshSTT182、SARS-CoV-1、RsYN04、PRD-0038与RmYN02的完整深度突变扫描数据集。本研究依托上述文库测定了多种表型特征，例如与ACE2同源物（ACE2 orthologs）或抗体的结合能力。