Flavobacterium sp. 40S8 Genome sequencing

Rhizosphere Grand Challenge Isolate Sequencing. The goal of this project is to comprehensively characterize the genomes of microorganisms found in the rhizosphere and endosphere of Arabidopsis thaliana. Organisms for study have been targeted based on their reproducible association with one or more Arabidopsis genotypes grown in natural soil. To obtain more complete genomes from the culturable fraction of the Arabidopsis microbiome, rhizosphere and endosphere samples were used to seed cultures on a variety of media. Cultures were screened by 16S rRNA sequencing and microbes of interest grown up and subjected to whole genome sequencing. Genomic sequences will be analyzed for genes participating in the plant-microbe symbiosis.根际大挑战分离株测序（Rhizosphere Grand Challenge Isolate Sequencing）。本项目旨在全面解析拟南芥（Arabidopsis thaliana）根际与内生境中微生物的基因组特征。研究对象的筛选依据为其与天然土壤中培育的一种或多种拟南芥基因型可重复的共生关联。为从拟南芥微生物组的可培养组分中获取更为完整的基因组，研究人员以根际与内生境样本为接种源，在多种培养基上进行培养。随后通过16S核糖体RNA（16S rRNA）测序对培养物进行筛选，挑取目标微生物进行扩增培养并开展全基因组测序。后续将针对参与植物-微生物共生（plant-microbe symbiosis）的基因，对所得基因组序列展开分析。