De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology

We report the use of RNA-seq data to assemble transcriptional units of adult Anopheles funestus female mosquitoes. We also analyzed expression levels and protein divergence and discovered SNPs. Overall design: Examination of full body mRNA from a single sample of 30 adult females. Center Name: Cornell-BPL. Fragments include median insert size of 103 bp, average insert size of 250 bp with a standard deviation of 22.

本研究利用RNA测序（RNA-seq）数据组装了成年雌性富氏按蚊（Anopheles funestus）的转录单元。此外，本研究还对基因表达水平、蛋白质分化情况进行了分析，并发现了单核苷酸多态性（SNPs，Single Nucleotide Polymorphisms）。实验设计：对由30只成年雌性蚊虫组成的单一样本的全身mRNA进行检测分析。项目中心名称：康奈尔-BPL（Cornell-BPL）。本研究使用的测序片段的插入片段大小中位数为103 bp（碱基对，base pair），平均插入片段大小为250 bp，标准差为22。