Effect of polymyxin B on simplexide- and LPS-induced release of IL-6, CXCL8 and TNF-α from monocytes.

Monocytes were incubated for 8 h (TNF-α) or 24 h (IL-6 and CXCL8) with simplexide (10 µM) or LPS (100 ng/ml) either in the absence (Control) or the presence of polymyxin B (50 µg/ml). Data are the mean ± SE of three experiments. * p<0.05 vs. respective untreated. †p<0.05 vs. respective control. Effect of polymyxin B on simplexide- and LPS-induced release of IL-6, CXCL8 and TNF-α from monocytes.将单核细胞与simplexide（10 μM）或脂多糖（Lipopolysaccharide, LPS，100 ng/ml）在有无多粘菌素B（Polymyxin B，50 μg/ml）的条件下孵育，其中肿瘤坏死因子-α（TNF-α）对应的孵育时长为8小时，白细胞介素6（IL-6）与趋化因子配体8（CXCL8）对应的孵育时长为24小时，对照组（Control）为未施加上述试剂的处理组。实验数据以三次独立重复实验的平均值±标准误（Standard Error, SE）表示。 * p<0.05 相较于相应未处理组；† p<0.05 相较于相应对照组。 多粘菌素B对simplexide与LPS诱导单核细胞释放IL-6、CXCL8及TNF-α的影响。