Measuring the impact of CSO discharge on microbial populations in an urban river.

This project exploits nanopore sequencing techniques to monitor water quality in the Ouse Burn river at Jesmond Dene in Newcastle-upon-Tyne, a popular leisure activity site shortly downstream of a combined sewer overflow. Water samples were taken periodically from July 2022 until May 2023 and microbial populations within samples were characterised via 16S barcoding PCR. Sequencing results were then cross-referenced with CSO discharge data to monitor the affect of discharging sewage on microbial populations within the river over time.

本项目采用纳米孔测序（nanopore sequencing）技术，对泰恩河畔纽卡斯尔市杰斯蒙德迪恩区域的乌斯伯恩河开展水质监测。该河段紧邻合流制下水道溢流（combined sewer overflow, CSO）的下游区域，同时也是一处热门休闲活动场地。研究团队于2022年7月至2023年5月期间定期采集水样，并通过16S条形码聚合酶链式反应（16S barcoding PCR）对样本内的微生物群落进行表征分析。随后将测序所得结果与合流制下水道溢流的排放数据进行交叉比对，以追踪污水排放随时间推移对河流内微生物群落造成的影响。