Transcriptional dynamics orchestrating the development and integration of neurons born in the adult hippocampus [ds2]. Transcriptional dynamics orchestrating the development and integration of neurons born in the adult hippocampus [ds2]

To profile neurogenesis in the adult mouse hippocampus, we utilized a strategy to isolate developing cells derived from RGLs expressing the proneural gene Achaete-scute homolog 1 (Ascl1). Tamoxifen administration to young-adult Ascl1CreERT2;CAGfloxStopSun1sfGFP mice were used to induce the expression of Sun1-sfGFP in the nuclear membrane of RGLs and permanently label their progeny. This birthdating tag enabled a comprehensive analysis of the entire process encompassing neuronal differentiation, development, and functional maturation. This dataset was built using FACS-sorted nuclei obtained from dentate gyri microdissected at 2-, 3-, 4-, 5- and 8 weeks after TAM induction (cohorts w2 through w8) Overall design: 10 samples

为了表征成年小鼠海马体的神经发生过程，我们构建了一套分离策略，用于分离源自表达前神经基因Achaete-scute同源物1（Ascl1）的放射状胶质样细胞（radial glia-like cells, RGLs）的发育中细胞。我们对年轻成年的Ascl1CreERT2;CAGfloxStopSun1sfGFP小鼠施用他莫昔芬，以诱导Sun1-sfGFP在放射状胶质样细胞的核膜中表达，并永久标记其所有子代细胞。该细胞诞生标记标签可实现对包含神经元分化、发育及功能成熟在内的完整过程的全面分析。本数据集采用经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）得到的细胞核构建而成，这些细胞核取自他莫昔芬诱导后第2、3、4、5和8周（即w2至w8队列）显微解剖获取的齿状回组织。实验整体设计包含10个样本。