A highly clonogenic subset of adult mouse biliary duct cells. Mus musculus

Our goal is to find the heterogeneity of mouse liver ductal cells in the wild type mouse liver. We separated the MIC1-1C3+ mouse liver ductal cells into two populations with the cell surface marker: ST14. We found that ST14hi1C3+ cells have higher colonal geneity comparing to the ST14lo1C3+ population. Overall design: Mouse liver NPCs were sorted by flowcytometry. The MIC1-1C3+CD26- non blood cells are seperated into two parts: ST14high and ST14low. Fresh sorted cells are put into TRIzol for RNA. RNA seq libraries were made with illumina TruSeq kit under the manufacture's protocol.

本研究旨在探究野生型小鼠肝脏中胆管细胞的异质性。我们以细胞表面标志物ST14为分型依据，将MIC1-1C3+小鼠胆管细胞分为两个亚群；研究发现，相较于ST14低表达（ST14lo）的1C3+细胞亚群，ST14高表达（ST14hi）的1C3+细胞亚群具有更高的克隆均一性。
整体实验设计：首先通过流式细胞术（flowcytometry）分选小鼠肝脏非实质细胞（non-parenchymal cells, NPC）；随后将其中的MIC1-1C3+CD26-非血液细胞分为ST14高表达组与ST14低表达组两个部分；将新鲜分选得到的细胞置于TRIzol试剂中提取RNA；按照厂商说明书，使用Illumina TruSeq建库试剂盒构建RNA测序文库。