pH Map of cryoablated MDR2 mouse

Mice were imaged 7 days post-cryoablation. MRI and MR Spectroscopic Imaging (MRSI) data were acquired using a 9.4T Bruker horizontal bore spectrometer. Anatomical T1-weighted images were obtained using a field of view (FOV) of 25mm x 25mm and 15 slices of 0.5 mm thickness. The MRSI datasets were reconstructed to a 19×15×25 matrix, corresponding to a 1mm³ isotropic voxel resolution. The pHe in each voxel was calculated in MATLAB (MathWorks, Inc., Natick, MA) from the 1H chemical shifts δ2, δ3, and δ6 of the H2, H3, and H6 protons of TmDOTP5-, respectively, as previously described. The MRSI signals were overlaid on the T1-weighted images for anatomical localization