pH Map of cryoablated MDR2 mouse

Mice were imaged 7 days post-cryoablation. MRI and MR Spectroscopic Imaging (MRSI) data were acquired using a 9.4T Bruker horizontal bore spectrometer. Anatomical T1-weighted images were obtained using a field of view (FOV) of 25mm x 25mm and 15 slices of 0.5 mm thickness. The MRSI datasets were reconstructed to a 19×15×25 matrix, corresponding to a 1mm³ isotropic voxel resolution. The pHe in each voxel was calculated in MATLAB (MathWorks, Inc., Natick, MA) from the 1H chemical shifts δ2, δ3, and δ6 of the H2, H3, and H6 protons of TmDOTP5-, respectively, as previously described. The MRSI signals were overlaid on the T1-weighted images for anatomical localization实验小鼠于冷冻消融术后7天接受成像。本研究采用9.4T布鲁克（Bruker）水平孔径波谱仪采集磁共振成像（Magnetic Resonance Imaging, MRI）与磁共振波谱成像（MR Spectroscopic Imaging, MRSI）数据。解剖学T1加权成像的采集参数为：视野（Field of View, FOV）25mm×25mm，共15层，层厚0.5mm。MRSI数据集被重建为19×15×25的矩阵，对应各向同性体素分辨率为1mm³。每个体素的细胞外pH（pHe）按照此前已报道的方法，通过MATLAB（美国马萨诸塞州纳蒂克市MathWorks公司）分别由TmDOTP5-的H2、H3和H6质子的1H化学位移δ2、δ3及δ6计算得到。将MRSI信号叠加于T1加权成像图像以实现解剖学定位。