IFN-γ Upregulates Survivin and Ifi202 Expression to Induce Survival and Proliferation of Tumor-Specific T Cells

Transcriptional profiling of mouse tumor-specific cytotoxic T lymphocytes (CTL) comparing activation-induced gene expression profiles induced by tumor-specific antigen (Ag) and CD3 mAb. CTLs were stimulated with either Ag or CD3 mAb for 3 and 24 h, respectively. The stimulated CTLs were compared to un-stimulated CTLs. The objective was to identify differential gene expression profiles induced by the two activation (Ag vs CD3 mAb) conditions. Two-condition experiments, un-stimulated vs stimulated CTLs. Biological replicates: 3 replicates for each comparison. Unstimulated CTLs were compared to stimulated CTLs (Ag and CD3 mAb, respectively).

本数据集针对小鼠肿瘤特异性细胞毒性T淋巴细胞（cytotoxic T lymphocytes, CTL）开展转录谱分析，比较肿瘤特异性抗原（Ag）与CD3单克隆抗体（CD3 mAb）所诱导的激活相关基因表达谱。实验分别以肿瘤特异性抗原、CD3单克隆抗体刺激CTL，其中抗原刺激组的刺激时长为3小时，CD3单克隆抗体刺激组的刺激时长为24小时。将受刺激的CTL与未受刺激的CTL进行对照，本研究旨在识别两种激活条件（肿瘤特异性抗原 vs CD3单克隆抗体）所诱导的差异基因表达谱。本实验包含两类对照组合：未受刺激CTL与受刺激CTL，每个对照组合均设置3次生物学重复。未受刺激CTL分别与抗原刺激组、CD3单克隆抗体刺激组的CTL进行对照比较。