A single cell RNA-Seq study of splenic marginal zone lymphomas (SMZL). A single cell RNA-Seq study of splenic marginal zone lymphomas (SMZL)

Purpose: Single cell RNA-Seq was used to characterize in depth splenic samples obtained from splenic marginal zone lymphomas Methods: 3 frozen samples of spleen from SMZL patients were selected and thawed for performing a 10X genomics single cell RNA-Sequencing 3' assay. Results: Tumor cells formed mainly patient specific-B cell clusters, while T-cells from the 3 patients clustered together. Conclusion: Single RNA-Seq unveils the cellular composition of splenic tumors from SMZL patients. Overall design: Frozen cell suspensions obtained from diagnosed splenic marginal zone lymphomas were selected for injection in the 10X chromium device for droplet-based RNA extraction, reverse transcription and cDNA amplification. Libraries were made with the Chromium i7 sample index plate (GENO55823). Sequencing was performed on a NextSeq 550 (Illumina).

研究目的：采用单细胞RNA测序（single cell RNA-Seq, scRNA-seq）深度表征源自脾边缘区淋巴瘤（splenic marginal zone lymphoma, SMZL）患者的脾脏样本。
研究方法：选取3份来自SMZL患者的脾脏冰冻样本，解冻后开展10X Genomics单细胞RNA测序3'端检测实验。
研究结果：肿瘤细胞主要形成患者特异性B细胞簇，而3名患者的T细胞则聚为统一集群。
研究结论：单细胞RNA测序可揭示SMZL患者脾脏肿瘤的细胞组成。
整体实验设计：将确诊为脾边缘区淋巴瘤的患者样本制备为冰冻细胞悬液，通过10X Chromium系统进行液滴法RNA提取、反转录及cDNA扩增；采用Chromium i7样本索引板（GENO55823）构建测序文库；测序在NextSeq 550（Illumina）测序平台上完成。