CIL:36018, damselfly, flight muscle cell. In Cell Image Library

Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.

Technical details:
Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.

Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199

该图像包含纵切面与横切面，可见带状肌原纤维（ribbon-like myofibrils）与线粒体（mitochondria）及肌浆网/横小管（SR/T tubules）层规律性交替排布。二联体（dyads）定位于线粒体表面的凹陷区域。底片经尼康（Nikon）8000扫描仪以4000dpi/6.3微米扫描步长扫描，随后将图像尺寸缩小4倍以用于上传，最终有效像素尺寸为25微米。

技术细节：
将样本去除头尾后，将胸部分为两半并浸没于0.1M二甲胂酸盐缓冲液（pH7.2）配制的3%戊二醛固定液中，于4℃下保存数日。取出肌肉组织进行解剖，用缓冲液漂洗3次，随后以缓冲液配制的2%四氧化锇（OsO4，原文疑似笔误为PsO4）后固定1.5小时；再用去离子水漂洗3次，采用饱和醋酸铀水溶液进行块染，最后包埋于Spurr树脂中。对超薄切片进行醋酸铀及铅染液染色后，使用飞利浦（Philips）410型显微镜进行观察。

图像参考文献：
Loesser, K.E.、Castellani, L. 及 Franzini-Armstrong, C. 《无脊椎动物肌肉中连接足的排布》，《肌肉研究与细胞运动》（J. Muscle Research Cell Motility）13卷：161-173，199