Estimation of genomic DNA contribution to RT-qPCR signal using −RT controls.

Quantitative PCR (qPCR) results obtained from reactions performed in the presence (+RT) or absence (−RT) of reverse transcriptase for 6 target genes. For +RT and −RT conditions, Cq values from three technical replicates (Cq1–Cq3) are shown along with the mean ± SD. The ΔCq value corresponds to the difference between −RT and +RT mean Cq values (ΔCq=(−RT) − (+RT)). Relative signal attributable to genomic DNA (gDNA) contamination was estimated as 2(-ΔCq). The contribution from −RT (%) represents the percentage of amplification signal arising from gDNA. NA indicates reactions with no detectable amplification. (XLSX)本数据集包含6个靶基因在添加（+RT）或不添加（-RT）逆转录酶（reverse transcriptase）的反应体系中获得的实时定量聚合酶链反应（Quantitative PCR, qPCR）结果。针对+RT与-RT两组实验条件，分别给出了3次技术重复的Cq值（Cq1–Cq3）以及均值±标准差（standard deviation, SD）。ΔCq值定义为-RT组与+RT组的平均Cq值之差，即ΔCq = (-RT组平均Cq) − (+RT组平均Cq)。可归因于基因组DNA（gDNA）污染的相对信号通过2^(-ΔCq)进行估算。-RT组扩增信号占比（%）代表了由基因组DNA所产生的扩增信号所占百分比。NA表示未检测到扩增信号的反应体系。数据文件格式为XLSX。