Direct Induction of Trophoblast Stem Cells from Human Fibroblasts (RRBS)

Transcription profile analysis revealed similarity/dissimilarity between human blastocyst derived trophoblast stem cells (hbdTSCs), human induced trophoblast stem cells (hiTSCs), human foreskin fibroblasts (HFFs) and pluripotent stem cells (hESCs, hiPSCs) Overall design: HFFs reprogrammed into hiTSC lines by transient ectopic expression of GATA3, OCT4, KLF4, MYC (GOKM) were subjected to RRBS-protocol to assess differentially methylated regions, along with samples of HFFs, hbdTSCs, hESCs and hiPSCs

转录谱分析揭示了人类囊胚来源滋养层干细胞（human blastocyst derived trophoblast stem cells, hbdTSCs）、人类诱导型滋养层干细胞（human induced trophoblast stem cells, hiTSCs）、人类包皮成纤维细胞（human foreskin fibroblasts, HFFs）与多能干细胞（pluripotent stem cells, hESCs、hiPSCs）之间的相似性与差异。实验设计概述：通过瞬时异位表达GATA3、OCT4、KLF4、MYC（GOKM组合）将HFFs重编程为hiTSC细胞系，随后将该细胞系与HFFs、hbdTSCs、hESCs及hiPSCs样本一同采用RRBS实验方案，以评估差异甲基化区域。