SMN protein timecourse across Delta7 mouse genotypes with statistical significance.

SMN protein values generally decline from P3 to P9 and P14 across all genotypes, though the rate of decline is distinct between genotypes. In general SMN protein levels in brain were higher than levels in spinal cord and muscle across all ages and genotypes. An ANOVA with Tukey's post-test performed for all analyses with the exception of the WT and HET comparisons at P14 that were done with an unpaired t-test as at P14 KO muscle signal was below the assay LOD.
*Denotes SMN protein values are significantly less statistically than the value of the same tissue at the previous age.
+Denotes SMN protein values are significantly less statistically than the value of the brain tissue of the same genotype and age. P-values are indicated by asterisks or plus signs in the following manner: p<0.001 by ***, p<0.01 by** and p<0.05 by *. LOD = limit of detection.

所有基因型的运动神经元生存蛋白（SMN protein）水平均在P3至P9、P14阶段呈下降趋势，但不同基因型的下降速率存在显著差异。总体而言，在所有年龄阶段与基因型中，脑组织中的SMN蛋白水平均高于脊髓与肌肉组织。所有分析均采用单因素方差分析（ANOVA）结合Tukey事后检验，仅P14阶段野生型（Wild Type, WT）与杂合子（Heterozygous, HET）的比较除外——因该阶段敲除型（Knockout, KO）肌肉的信号低于检测限（LOD），故该组比较采用非配对t检验完成。
* 表示SMN蛋白水平较相同组织、相同基因型的前一年龄阶段的水平显著降低。
+ 表示SMN蛋白水平较相同基因型、相同年龄阶段的脑组织水平显著降低。显著性P值通过星号与加号标注规则如下：***对应p<0.001，**对应p<0.01，*对应p<0.05。LOD即检测限（limit of detection）。