Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator [RNA-seq]

EZH2 induces active transcription of the AR gene, thereby increasing AR level and promoting AR signaling. Importantly, EZH2-mediated activation of AR requires EZH2 protein occupancy at the AR gene promoter, but is independent of PRC2 as well as its histone methyltransferase activity RNA-seq examination of combinatorial effects of ENZ+EPZ in prostate cancer cells signaling pathwayEZH2可诱导雄激素受体（AR）基因的活跃转录，进而提升AR蛋白水平并促进AR信号通路活化。值得注意的是，EZH2介导的AR激活需要EZH2蛋白结合于AR基因启动子区域，但该过程不依赖于PRC2及其组蛋白甲基转移酶活性。本研究通过RNA测序（RNA-seq）分析了ENZ+EPZ联合处理对前列腺癌细胞信号通路的联合效应。