Arabidopsis SE coordinates histone methyltransferases ATXR5/6 and RNA processing factor RDR6 to regulate transposon expression [sRNA-Seq]

We performed Illumina sequencing of sRNA libraries prepared from 10-day-old seedlings in Arabidopsis. SE is a key component in miRNA processing and pre-mRNA splicing, whereas ATXR5/6 is engaged in epigenetic silencing. Genome-wide sRNA sequencing revealed that ATXR5/6 did not participate in SE-mediated miRNA biogenesis. Non-coding RNA profiling by high throughput sequencing.

本研究对拟南芥（Arabidopsis）10日龄幼苗制备的sRNA（small RNA）文库进行了Illumina测序（Illumina sequencing）。SE是miRNA（microRNA）加工与pre-mRNA剪接的关键组分，ATXR5/6则参与表观遗传沉默（epigenetic silencing）过程。全基因组sRNA测序结果显示，ATXR5/6并未参与SE介导的miRNA生物发生过程。本数据集通过高通量测序（high throughput sequencing）完成了非编码RNA（non-coding RNA）的表达谱分析。