Performance of RcsF mutant library in three genetic screens.

Data was collected and analyzed as described in Materials and Methods. “Reads (sum)” represents a sum of non-identical nucleotides reads encoding the same a.a. variant. Stop codon indicated by “*”. “Read fraction” represents a Read Sum normalized by a total nucleotide read count in that NGS sample. “Read fraction” was used for log2[FC] calculations. If the variant was not detected post-selection, we used relative abundance corresponding to 2 reads (below the threshold) to facilitate the log2[FC] calculation. (XLSX)本研究数据按照材料与方法部分所述流程完成采集与分析。"Reads（总和）（Reads (sum)）"代表编码同一氨基酸（amino acid，a.a.）变异体的非冗余核苷酸测序读段之和。终止密码子以"*"符号标注。"读段占比（Read fraction）"指以该下一代测序（Next-Generation Sequencing，NGS）样本的总核苷酸测序读段数对Reads（总和）进行归一化后所得的数值。"读段占比"用于对数2[倍数变化（fold change，FC）]值的计算。若筛选后未检测到该变异体，则采用对应2条读段的相对丰度（低于检测阈值）以辅助完成对数2[倍数变化]值的计算。(XLSX)