Metagenome-assembled genomes from Wind River Basin floodplain sediments Riverton, Wyoming site (June to October 2019)

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken at three time points from June 12, 2019 to October 23,2019 at a location (PTT1) close to DOE Legacy Management well 855 at the Riverton, Wyoming floodplain site in the Wind River Basin (WRB). The groundwater at this site exhibits persistent U, Mo, and sulfate plumes and is one of the field sites in focus for the SLAC Groundwater Quality SFA program. Sediment samples were collected from 60 to 180 cm below surface every 30cm for microbial analyses through metagenomic sequencing. 15 metagenomes were sequenced through JGI and can be found under Gold sequencing project: Gs0131241. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 780 MAG fasta files and a csv file with quality, taxonomic classification (GTDB RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. A sample metadata file (samples.csv) that contains site information has also been included.

微生物凭借自身遗传潜力，在陆地环境的营养物与污染物循环过程中发挥关键作用。本研究公开了细菌与古菌群落的宏基因组组装基因组（metagenome-assembled genomes，MAGs），样品采集自2019年6月12日至2019年10月23日三个时间点下，位于怀俄明州里弗顿风河流域（WRB）洪泛区场地、靠近美国能源部（Department of Energy，DOE）遗产管理局855号井的PTT1点位的洪泛沉积物。该场地的地下水中存在持续留存的铀（U）、钼（Mo）与硫酸盐羽流，是SLAC地下水质量SFA项目重点关注的野外场地之一。研究人员于地表下60至180 cm范围内以30 cm为间隔采集沉积物样品，用于宏基因组测序的微生物组分析。15个宏基因组由联合基因组研究所（Joint Genome Institute，JGI）完成测序，相关数据可在基因组在线数据库（Genomes Online Database，GOLD）测序项目Gs0131241中获取。研究团队使用MetaWRAP工具对宏基因组进行组装、分箱与优化，最终得到符合CheckM评分标准的MAGs（完整度>50%、污染率<10%）。本数据集包含780个MAG的FASTA格式文件压缩包，以及一份记录MAG质量、分类学注释（基于基因组分类数据库（Genome Taxonomy Database，GTDB）RS220标准）与宏基因组登录号的CSV文件。本数据集还包含文件级元数据文件flmd.csv，该文件列出数据集内所有文件及其关联元数据；另有数据字典文件dd.csv，其中记录了所有文件中使用的行列表头，及其定义、单位与数据类型。此外还附带一份包含场地信息的样品元数据文件samples.csv。