Comprehensive transcriptomes of mouse pancreatic beta cells that transdifferentiated from alpha cells. Mus musculus

Rodent models are widely used to study diabetes. Yet, significant gaps remain in our understanding of mouse islet physiology, including the maintenance of mature endocrine cell fate. We generated comprehensive transcriptomes beta cells that transdifferentiated from alpha cells using a triple transgenic mouse models generated for this purpose. Overall design: FACS purified beta cells with an alpha cell lineage label that reflect beta cells that transdifferentiated from alpha cells, along with alpha cells and beta cells [GSE80673] from the same islets. Islets were isolated from triple transgenic offspring of a cross between Ins1-H2b-mCherry (Jax # 028589) and Gcg-cre (alpha) cells and a floxed YFP allele to lineage-label alpha cells. Islets from replicate groups of 10 to 12 triple transgenic animals for each group were pooled by sex to obtain sufficient material. Pooled islets were dissociated, sorted and collect in Trizol for RNA isolation and library construction. Islets from replicate groups of 10 to 12 bitransgenic females for each group were pooled to obtain sufficient material. Pooled islets were dissociated, sorted and collect in Trizol for RNA isolation and library construction.

啮齿类动物模型（rodent models）被广泛应用于糖尿病研究领域。然而，当前学界对小鼠胰岛生理学的认知仍存在显著空白，其中涵盖成熟内分泌细胞命运的维持机制。本研究利用专为该实验构建的三重转基因小鼠模型（triple transgenic mouse models），对由α细胞转分化而来的β细胞生成了全面的转录组（transcriptome）数据。
实验设计概述：采集经荧光激活细胞分选（FACS）纯化的、带有α细胞谱系标记的β细胞——该标记可反映由α细胞转分化得到的β细胞——同时采集同一胰岛中的α细胞与β细胞 [GSE80673]。
胰岛分离自以下杂交组合的三重转基因子代小鼠：Ins1-H2b-mCherry（Jax品系编号#028589）、Gcg-cre（α细胞标记）小鼠，以及携带可对α细胞进行谱系标记的floxed YFP等位基因的小鼠。每个重复组取10至12只三重转基因小鼠，按性别合并胰岛以获取足够实验材料；合并后的胰岛经解离、分选后，收集于Trizol试剂中用于RNA提取与文库构建。
此外，每个重复组取10至12只双转基因雌性小鼠，合并胰岛以获取足够实验材料；合并后的胰岛经解离、分选后，收集于Trizol试剂中用于RNA提取与文库构建。