"Human visual cortex responses to rapid cone and melanopsin-directed flicker" (Spitschan et al.) – Data Supplement

"Human visual cortex responses to rapid cone and melanopsin-directed flicker" (Spitschan et al.)

Data Supplement

Figures 

Figure S1 [Figure_S1.pdf] | Echoplanar image intensity. Representative echoplanar images for each subject. The three views (sagittal, horizontal, and coronal) also include a colored overlay of the visual area ROIs (V1 and V2/V3, not restricted in eccentricity range). Image intensity is the mean of the set of spatially-aligned echoplanar images from a single scanning run and reflects signal-to-noise for a given spatial location. ROIs defined on the cortical surface were projected back to the volumetric space using FreeSurfer.

Figure S2 [Figure_S2.pdf] | Psychophysical nulling (average). a, Perceptual nulling data for a 32% melanopsin (Mel*; non penumbral-cone silent) modulation in a population of subjects (n=15). Primary subjects (S01, S02, and S03) indicated with a star symbol. Ellipse indicates ±1SD across subjects. Some plot points are overlapping. b, Perceptual nulling data for a 32% cone directed (L+M+S) modulation in the same population of 15 subjects. Red error bar indicates ±1SD across subjects. Averages were obtained from the positive and negative arms shown in Fig. 3. All data are tabulated in Table S6.

Figure S3 [Figure_S3.pdf] | V2/V3, MT and LOC response to melanopsin modulations. BOLD amplitudes shown as average across the two ROI hemispheres and across ROI vertices in the relevant eccentricity range (inset). Format follows Fig. 5.

Figure S4 [Figure_S4.pdf] | Additional BOLD data. BOLD amplitudes shown as average across the two V1 hemispheres and across V1 vertices in the relevant eccentricity range (3°-13°). Subjects viewed 12 sec 4 Hz flicker with a dilated pupil. Experimental details and data analysis follows main experiments. Melanopsin and L+M+S modulations were nulled prior to the experiment. All modulations at ±32% contrast. Data shown were not included in the main report because of 1) equipment failure, preventing us from collecting behavioral responses during the attention task, 2) concern that subject S03 was sleeping in the scanner per his self report, 3) failure of the positive control modulation (light flux) to evoke a response in subject S03, and 4) the difficulty of interpreting any observed response to the melanopsin modulation, given that the modulation also stimulated the penumbral cones.

Tables

Table S1 [Table_S1.xlsx] | Spreadsheet of spectral power distributions. All modulations are unsigned difference spectra. To derive the displayed stimulus, they would be added to the background. Spectral power distributions have been splined to 1 nm wavelength spacing (measured at 2 nm wavelength bands).

Table S2 [Table_S2.xlsx] | Spreadsheet of stimulus sequences (fMRI experiments). Within a given BOLD fMRI run, subjects viewed 12 s segments. The order of frequencies was counterbalanced and is given in the columns. 

Table S3 [Table_S3.xlsx] | Spreadsheet of stimulus sequences (pupil experiments). Within a given pupillometry run, subjects viewed 45 sec trials. The sequence is given in the rows, modulations and run types per column. 

Table S4 [Table_S4.xlsx] | Demographic details about subjects.

Table S5 [Table_S5.xlsx] | Individual subject pupil measures. Corresponds to data shown in graphical form in Fig. 3b.

Table S6 [Table_S6.xlsx] | Individual subject nulling measures. Corresponds to data shown in graphical form in Fig. 3a. Sheet 1 contains the nulling values for the ±32% L+M+S and melanopsin modulations (subjects S01-15). Sheet 2 contains the nulling values for the ±17% melanopsin modulations (subjects S01-03).

Data
Data S1 [Data_S1.tar.gz] | BOLD fMRI Data (L+M, L-M, S, 17% melanopsin, 2-64 Hz). Beta effect size maps from each subject (as well as the mean EPI image), registered to the whole-brain, individual anatomical image in native subject space. See README.txt for details. [MD5 (Data_S1.tar.gz) = c17d1e8f872f78d2f7feefa860e7794d].
Data S2 [Data_S2.tar.gz] | BOLD fMRI Data (L+M, L-M, S, 17% melanopsin, 0.5-2 Hz). Beta effect size maps from each subject (as well as the mean EPI image), registered to the whole-brain, individual anatomical image in native subject space. See README.txt for details. [MD5 (Data_S2.tar.gz) = ce06eae027f0e550e077faf06871f27b].
Data S3 [Data_S3.tar.gz] | BOLD fMRI Data (L+M+S, 0.5-64 Hz). Beta effect size maps from each subject (as well as the mean EPI image), registered to the whole-brain, individual anatomical image in native subject space. See README.txt for details. [MD5 (Data_S3.tar.gz) = 8aa43a415a84bc09b56dbec8aa038e13].
Data S4 [Data_S4.tar.gz] | BOLD fMRI Data (L*+M*, 2-64 Hz; scaled L+M, 0.5-64 Hz). Beta effect size maps from each subject (as well as the mean EPI image), registered to the whole-brain, individual anatomical image in native subject space. See README.txt for details. [MD5 (Data_S4.tar.gz) = 8e8239784c5932ee7543cc335bdae046].
Data S5 [Data_S5.tar.gz] | BOLD fMRI Data (Light flux, nulled and un-nulled melanopsin, L+M+S and L-M nulling contrast; 4 Hz). Beta effect size maps from each subject (as well as the mean EPI image), registered to the whole-brain, individual anatomical image in native subject space. See README.txt for details. [MD5 (Data_S5.tar.gz) = 8b377ab28e99bf9eb9fbe8cb440ffc1c].

人类视觉皮层对快速视锥细胞与黑视素靶向闪烁的响应（Spitschan等）

数据补充材料

图 片
图S1 [Figure_S1.pdf] | 回波平面成像强度。展示每位受试者的典型回波平面成像图像。三种视图（矢状位、水平位、冠状位）同时叠加了视觉皮层感兴趣区（Region of Interest, ROI）——V1与V2/V3，未限制偏心范围——的彩色标记。图像强度为单次扫描序列中空间配准的回波平面成像集合的均值，反映了特定空间位置的信噪比。基于皮层表面定义的感兴趣区通过FreeSurfer软件投影回体素空间。

图S2 [Figure_S2.pdf] | 心理物理学零点校正（平均结果）。a，针对15名受试者群体中32%黑视素（Mel*；非半影视锥静默）调制的知觉零点校正数据。以星号标记核心受试者（S01、S02、S03）。椭圆代表受试者间的±1标准差。部分绘图点存在重叠。b，同一15名受试者群体中针对32%视锥靶向（L+M+S）调制的知觉零点校正数据。红色误差棒代表受试者间的±1标准差。平均值取自图3所示的正负两组实验条件。所有数据均整理于表S6中。

图S3 [Figure_S3.pdf] | V2/V3、MT与LOC脑区对黑视素调制的响应。展示了双侧ROI半球以及对应偏心范围（内嵌图）内ROI顶点的血氧水平依赖（Blood Oxygen Level Dependent, BOLD）振幅平均值。图表格式与图5一致。

图S4 [Figure_S4.pdf] | 额外BOLD数据。展示了双侧V1半球以及对应偏心范围（3°-13°）内V1顶点的BOLD振幅平均值。受试者观看时长12秒、频率4Hz的闪烁刺激，且瞳孔处于散大状态。实验细节与数据分析遵循主实验流程。实验前已对黑视素与L+M+S调制进行零点校正。所有调制的对比度均为±32%。本部分数据未纳入主报告，原因如下：1）设备故障，无法在注意任务期间收集行为学响应；2）受试者S03自述在扫描过程中处于睡眠状态，引发顾虑；3）阳性对照调制（光通量）未能在受试者S03中诱发出响应；4）鉴于该黑视素调制同时刺激了半影视锥，难以解释观测到的黑视素调制相关响应。

表 格
表S1 [Table_S1.xlsx] | 光谱功率分布电子表格。所有调制均为无符号差分光谱。若要生成展示的刺激，需将其叠加至背景光中。光谱功率分布已通过样条插值处理为1nm波长间隔（原始测量波段为2nm）。

表S2 [Table_S2.xlsx] | 刺激序列电子表格（功能磁共振成像实验）。在单次血氧水平依赖功能磁共振成像（BOLD fMRI）扫描序列中，受试者观看时长12秒的片段。刺激频率的顺序采用平衡设计，具体信息列于各列中。

表S3 [Table_S3.xlsx] | 刺激序列电子表格（瞳孔测量实验）。在单次瞳孔测量扫描序列中，受试者观看时长45秒的试次。刺激序列按行排列，各列分别对应调制类型与扫描序列类型。

表S4 [Table_S4.xlsx] | 受试者人口统计学细节信息。

表S5 [Table_S5.xlsx] | 受试者个体瞳孔测量数据。对应图3b以图表形式展示的数据。

表S6 [Table_S6.xlsx] | 受试者个体零点校正数据。对应图3a以图表形式展示的数据。工作表1包含±32% L+M+S与黑视素调制的零点校正值（受试者S01-15）。工作表2包含±17%黑视素调制的零点校正值（受试者S01-03）。

数 据
数据S1 [Data_S1.tar.gz] | BOLD fMRI数据（L+M、L-M、S、17%黑视素，2-64Hz）。包含每位受试者的β效应量映射图（以及平均EPI图像），已配准至个体原生空间下的全脑解剖图像。详细信息参见README.txt。[MD5 (Data_S1.tar.gz) = c17d1e8f872f78d2f7feefa860e7794d]

数据S2 [Data_S2.tar.gz] | BOLD fMRI数据（L+M、L-M、S、17%黑视素，0.5-2Hz）。包含每位受试者的β效应量映射图（以及平均EPI图像），已配准至个体原生空间下的全脑解剖图像。详细信息参见README.txt。[MD5 (Data_S2.tar.gz) = ce06eae027f0e550e077faf06871f27b]

数据S3 [Data_S3.tar.gz] | BOLD fMRI数据（L+M+S，0.5-64Hz）。包含每位受试者的β效应量映射图（以及平均EPI图像），已配准至个体原生空间下的全脑解剖图像。详细信息参见README.txt。[MD5 (Data_S3.tar.gz) = 8aa43a415a84bc09b56dbec8aa038e13]

数据S4 [Data_S4.tar.gz] | BOLD fMRI数据（L*+M*，2-64Hz；缩放型L+M，0.5-64Hz）。包含每位受试者的β效应量映射图（以及平均EPI图像），已配准至个体原生空间下的全脑解剖图像。详细信息参见README.txt。[MD5 (Data_S4.tar.gz) = 8e8239784c5932ee7543cc335bdae046]

数据S5 [Data_S5.tar.gz] | BOLD fMRI数据（光通量、已校正与未校正黑视素、L+M+S与L-M校正对比度；4Hz）。包含每位受试者的β效应量映射图（以及平均EPI图像），已配准至个体原生空间下的全脑解剖图像。详细信息参见README.txt。[MD5 (Data_S5.tar.gz) = 8b377ab28e99bf9eb9fbe8cb440ffc1c]