Figure S1 - Expression of TIP-1 Confers Radioresistance of Malignant Glioma Cells

Impact of X-ray irradiation on the TIP-1 expression levels in D54 cells. (A) Monolayer of D54 cells were irradiated with variable doses of X-ray and cell lysates were prepared for SDS-PAGE and western blot profiling of protein expression at 12 hours post the irradiation. Mock (0 Gy) treated cells were used as control. (B) Monolayer of D54 cells were irradiated with 5 Gy of X-ray and cell lysates were prepared for SDS-PAGE and western blot profiling of protein expression at variable time points post the irradiation. Mock (0 Gy) treated cells were used as control. Actin was blotted in each sample.
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X射线辐照对D54细胞中TIP-1表达水平的影响。(A) 对单层培养的D54细胞进行不同剂量的X射线辐照，于辐照后12小时制备细胞裂解液，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）及蛋白质印迹（western blot）技术对蛋白表达进行分析。以0 Gy假处理（Mock）的细胞作为对照组。(B) 对单层培养的D54细胞进行5 Gy剂量的X射线辐照，于辐照后不同时间点制备细胞裂解液，采用SDS-PAGE及蛋白质印迹技术对蛋白表达进行分析。以0 Gy假处理的细胞作为对照组。所有样品均以肌动蛋白（Actin）作为内参进行免疫印迹检测。(PDF)