Wnt evolution and function shuffling in liberal and conservative chordate genomes

Additional file 1 (pdf file; 11.8 MB) includes: <b>Figure S1</b>. Evolution of Wnt5 in ascidians. <b>Figure S2</b>. Expression of WntA in two ascidian species. <b>Figure S3</b>. Chordate Wnt expression. <b>Figure S4</b>. Wnt subfamilies in <i>A. lucayanum</i>, <i>P. marinus</i> and <i>C. milii</i>. <b>Table S1</b>. Non-vertebrate chordate Wnt genes. <b>Table S2</b>. <i>Branchiostoma lanceolatum</i> and <i>Halocynthia roretzi</i> primer and probe sequences. <b>Table S3</b>. Genomic coordinates (scaffold or chromosome) for cephalochordate, human and lamprey <i>Wnt</i> genes. <b>Text S1</b>. <i>Branchiostoma lanceolatum Wnt</i> expression as shown in Figure 2. <b>Text S2</b>. References for Figure S3. Additional file 2 (fasta file; 315 KB): Wnt sequence alignement for Figure 1. Additional file 3 (fasta file; 102 KB): Wnt sequence alignment for Figure S4.附加文件1（PDF文件；11.8 MB）包含以下内容： **图S1**：被囊动物中Wnt5的进化历程 **图S2**：两种被囊动物的WntA表达模式 **图S3**：脊索动物Wnt基因表达情况 **图S4**：*A. lucayanum*、*P. marinus*与*C. milii*中的Wnt亚家族 **表S1**：非脊椎动物脊索动物的Wnt基因 **表S2**：*Branchiostoma lanceolatum*与*Halocynthia roretzi*的引物与探针序列 **表S3**：头索动物、人类及七鳃鳗的*Wnt*基因的基因组坐标（支架或染色体位置） **文本S1**：如图2所示的*Branchiostoma lanceolatum*的*Wnt*基因表达情况 **文本S2**：图S3的参考文献 附加文件2（FASTA文件；315 KB）：用于图1的Wnt序列比对文件 附加文件3（FASTA文件；102 KB）：用于图S4的Wnt序列比对文件