Induction of corneal collagen cross-linking in experimental corneal alkali burns in rabbits

Objective: To evaluate the effect of riboflavin-ultraviolet-A-induced cross-linking (CXL) following corneal alkali burns in rabbits. Methods: The right corneas and limbi of ten rabbits were burned using a 1N solution of NaOH and the animals were then divided into two groups: a control group submitted to clinical treatment alone and an experimental group that was treated 1 h after injury with CXL, followed by the same clinical treatment as administered to the controls. Clinical parameters were evaluated post-injury at 1, 7, 15, and 30 days by two independent observers. Following this evaluation, the corneas were excised and examined histologically. Results: There were no statistically significant differences in clinical parameters, such as hyperemia, corneal edema, ciliary injection, limbal ischemia, secretion, corneal neovascularization, symblepharon, or blepharospasm, at any of the time-points evaluated. However, the size of the epithelial defect was significantly smaller in the CXL group (p研究目的：评估核黄素-紫外线A诱导交联（CXL）对家兔角膜碱烧伤后的干预效果。 研究方法：采用1当量氢氧化钠溶液烧灼10只家兔的右眼角膜及角膜缘，随后将家兔分为两组：对照组仅接受单纯临床治疗；实验组于伤后1小时实施CXL治疗，后续采用与对照组一致的临床治疗方案。分别于伤后1、7、15及30天，由两名独立观察者对各项临床指标进行评估。评估完成后，摘除家兔角膜并进行组织学检查。 研究结果：在所有评估时间点中，两组在充血、角膜水肿、睫状充血、角膜缘缺血、分泌物情况、角膜新生血管、睑球粘连及眼睑痉挛等临床指标上均无统计学显著差异。但实验组的上皮缺损面积显著更小（p