Transcriptome of Anthurium andraeanum under cold stress

The transcriptome of leaves from two Anthurium cultivars, Oregon (Elegang, E, cold tolerant) and Fantasy love (Menghuan, MH, cold sensitive) under cold stress were sequenced by Illumina Novaseq™ 6000. Sequencing generated a total of 129.44 Gb of raw reads and an average of 7.19 Gb of reads for each sample. The analysis showed differences of transcriptomes between the control and the cold treatment samples. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles inAnthurium cold tolerance.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the cold stress response of A. andraeanum. Overall design: The full-flowering plants were grown in the growth chambers for three days with a 12/12 h day/night cycle at 25?. For cold treatments, the plants were grown at 15? for three days, then the temperature was decreased to 6? for three days and then 4? for three days. The control plants were grown at 25? throughout the experiment. Leaves were collected at 25?(control/CK), three days after the treatments at 6? and 4? for RNAseq. Three independent experimental replicates were performed, each consisting of three independent biological replicates.

本数据集对两个花烛（Anthurium）品种——耐寒品种俄勒冈（Elegang，代号E）与冷敏感品种梦幻恋（Menghuan，代号MH）的叶片在冷胁迫下的转录组（transcriptome）进行了测序，测序平台为Illumina Novaseq™ 6000。本次测序共产出129.44 Gb原始读段，每个样本的平均读段数据量为7.19 Gb。

分析结果显示，对照组与冷胁迫处理组的叶片转录组存在显著差异。本研究共鉴定出大量差异表达基因（differentially expressed genes, DEGs），这些基因呈现出各异的表达模式。此类基因在花烛耐寒性调控中已被证实或潜在发挥功能，因此可作为进一步研究红掌（A. andraeanum）冷胁迫响应相关基因表达与调控机制的理想候选对象。

整体实验设计：
将盛花期植株置于光照/黑暗周期为12/12 h、温度25℃的人工气候箱中预培养3天。冷胁迫处理组的培养流程为：先在15℃下培养3天，随后降温至6℃培养3天，最后降至4℃继续培养3天。对照组植株在整个实验期间均维持25℃培养。分别在25℃（对照组/CK）、6℃处理3天以及4℃处理3天后采集叶片，用于RNA测序（RNAseq）。本实验共设置3次独立实验重复，每次实验重复包含3份独立生物学重复样本。