Noncoding RNA HID1 promotes phyB-dependent seed germination by repressing the biosynthesis of ABA [ChIP-seq]

Arabidopsis noncoding RNA HID1 acts as a positive regulator to promote phyB-mediated seed germination by modulating the biosynthesis of abscisic acid. Overall design: Three biological replicates of each genotype were used for high-throughput sequencing analysis. For seed germination-related experiments, all plants were grown side-by-side, and the seeds were harvested on the same day. The seeds were dried at room temperature for about 2 months prior to subsequent experiments. Including sterilization and plating, seeds were exposed to white light for 1 hour. For the phyB-off condition, the seeds were exposed to far-red light (5 µmol·m-2·s-1) for 5 min to inactivate phyB. For the phyB-on condition, the seeds were irradiated with red light (20 µmol·m-2·s-1) for 5 min to activate phyB. The seeds were then incubated in the dark for the indicated time, and the germination rates were determined in the dark room.

拟南芥（Arabidopsis）非编码RNA（noncoding RNA）HID1作为正向调控因子，通过调控脱落酸（abscisic acid）的生物合成，促进光敏色素B（phyB）介导的种子萌发。
实验设计：每个基因型设置三次生物学重复，用于高通量测序分析。针对种子萌发相关实验，所有植株均同期平行种植，并于同一天收获种子。后续实验前，种子于室温下干燥约2个月。包括灭菌与铺板环节在内，种子均经1小时白光照射处理。设置光敏色素B失活组时，将种子置于5 µmol·m⁻²·s⁻¹的远红光下照射5分钟以灭活光敏色素B；设置光敏色素B激活组时，将种子以20 µmol·m⁻²·s⁻¹的红光照射5分钟以激活光敏色素B。随后将种子于黑暗条件下孵育至指定时长，随后在暗室中统计种子萌发率。