Gene expression profile at single cell level of mouse heart from control diet (CD), high fat diet (HFD) and Western diet (WD)

Macrophages are considered as a homogeneous population in the heart. We used single cell RNA sequencing (scRNA-seq) to analyze thediversity of macrophages in control diet (CD) mouse, high fat diet (HFD) mouse and Western diet (WD) mouse heart. Overall design: CD45+F4/80+CD11b+ macrophages of the diet mice were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq.

巨噬细胞（Macrophages）曾被认为是心脏内的均质细胞群体。本研究采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq），分析对照饮食（control diet, CD）、高脂饮食（high fat diet, HFD）及西式饮食（Western diet, WD）干预小鼠的心脏巨噬细胞多样性。整体实验设计：通过荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）分离上述三组饮食干预小鼠的CD45+F4/80+CD11b+巨噬细胞，随后采用单细胞RNA测序完成分析。