Proteomic and Genomic Analyses of the Rvb1 and Rvb2 Interaction Network upon Deletion of R2TP Complex Components. Saccharomyces cerevisiae

The Saccharomyces cerevisiae R2TP protein complex consists of Rvb1, Rvb2, Pih1 and Tah1. The R2TP complex has been implicated in various cellular processes such as assembly of snoRNP complex, RNA polymerase II complex, apoptosis and PIKK signaling. The involvement of R2TP in assembling various complexes seems to be in part due to Pih1 and Tah1, which serve as adapter/recruiter proteins. Here, we have performed high resolution RNA-seq. analyses to identify differential expression levels between wild type and PIH1 and TAH1 deletion strains of Saccharomyces cerevisiae that can help in unraveling other functions of Pih1 and Tah1. Both wild type and deletion strains contained TAP (tandem affinity purification) tag at the C-terminal end of either RVB1 or RVB2. Overall design: 3 biological replicates were performed for each strains

酿酒酵母(Saccharomyces cerevisiae) R2TP蛋白复合物由Rvb1、Rvb2、Pih1和Tah1组成。该复合物已被证实参与多种细胞进程，包括snoRNP（小核仁核糖核蛋白，small nucleolar ribonucleoprotein）复合物、RNA聚合酶II复合物的组装，细胞凋亡以及PIKK（磷脂酰肌醇3-激酶相关激酶，phosphatidylinositol 3-kinase-related kinase）信号通路。R2TP复合物介导多种复合物组装的特性，在一定程度上依赖于作为衔接/招募蛋白的Pih1和Tah1。本研究通过高分辨率RNA测序(RNA-seq)分析，鉴定酿酒酵母野生型菌株与PIH1、TAH1基因缺失菌株之间的差异表达水平，以期阐明Pih1和Tah1的其他未知功能。所有野生型菌株与基因缺失菌株均在RVB1或RVB2的C端携带TAP（串联亲和纯化，tandem affinity purification）标签。实验设计：每个菌株设置3次生物学重复。