Data segmented wings
收藏资源简介:
Data for the paper "Signalling-dependent refinement of cell fate choice during tissue remodelling". The folders "Movie200923" and "Dumpy_DSRF_1" store data necessary for running simulations on wing tissues, such as cell centroids, cell lineages, cell neighbours, and so on. They contain wing images with cell junctions labelled by E-cadherin, which have been segmented and tracked. They also contain raw images of DSRF>GFP fluorescence. "200923" contains data from the wild type wing; "200924_wing1" contains data from the Dumpy mutant wing. The database "DB.csv" ("DBcells2.csv" for the Dumpy mutant) also stores the DSRF level in each cell at all time points. To run the simulation, it is sufficient to save the folder Movie200923.zip (Dumpy_DSRF_1.zip) in the same directory as "Run_sim_WT_wing.py" (Run_sim_Dumpy_wing.py).
本数据集配套于论文《组织重塑过程中依赖信号传导的细胞命运选择精细化调控》。文件夹“Movie200923”与“Dumpy_DSRF_1”存储了翅组织模拟所需的各类数据,包括细胞质心、细胞谱系、细胞邻接关系等。其中包含经分割与追踪处理、以E-钙黏蛋白(E-cadherin)标记细胞连接的翅组织图像,同时还包含以DSRF驱动绿色荧光蛋白(GFP)表达的原始荧光图像。其中“200923”文件夹存储野生型翅组织的数据;“200924_wing1”文件夹存储Dumpy突变型翅组织的数据。数据库文件“DB.csv”(Dumpy突变型对应数据库为“DBcells2.csv”)同时存储了所有时间点下每个细胞的DSRF表达水平。若要运行模拟程序,仅需将文件夹Movie200923.zip(或Dumpy_DSRF_1.zip)与对应脚本“Run_sim_WT_wing.py”(或“Run_sim_Dumpy_wing.py”)置于同一目录下即可。



