Ambiguous sequences at different cut off levels - (Item E)
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For the evaluation of ambiguous sequences in microbial reference genomes we assessed different cut of levels for the generation of a curated microbial genome database. For each sequence fragment we determined the ratio between the number of negative BLAST hits (query genus ≠ subject genus) and all BLAST hits.<br><br>For a detailed description of this analysis, see the Supplemental Materials section of the publication.<br><br><b>Reference</b> <b>Kirstahler P, Bjerrum SS, Friis-Møller A, </b><b>la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018) </b>Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.<b> </b><b>Scientific Reports</b>, doi:10.1038/s41598-018-22416-4.
为评估微生物参考基因组中的模糊序列，我们针对经人工精选的微生物基因组数据库的构建流程，评估了不同的截断阈值。针对每一段序列片段，我们计算了阴性BLAST（Basic Local Alignment Search Tool）比对结果（查询序列所属属与比对目标序列所属属不相符）的数量与总BLAST比对结果数量之间的比值。 有关本次分析的详细说明，请参阅该论文的补充材料章节。 <b>参考文献</b> <b>Kirstahler P, Bjerrum SS, Friis-Møller A, la Cour M, Aarestrup FM, Westh H., and Pamp SJ. (2018)</b> 《基于基因组学的人类眼房液微生物鉴定》。<b> </b><b>《科学报告（Scientific Reports）》</b>，doi:10.1038/s41598-018-22416-4.
