Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation II. Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation II

M17 and HOG cells were induced differentiation and subject to A-tailing RNaseR treatment, circRNA were identified by rRNA depleted RNA-seq data from A-tailing RNaseR and untreated samples Overall design: M17, HOG, M17dif, HOGdif, each group with 3 replicates. A-tailing RNaseR and untreated library for each sample.

对M17细胞与HOG细胞实施诱导分化后，分别进行腺苷酸加尾（A-tailing）与核糖核酸酶R（RNaseR）处理；本研究采用经上述处理及未处理样本的核糖体RNA（rRNA）去除型RNA测序（RNA-seq）数据，完成环状RNA（circRNA）的鉴定工作。整体实验设计如下：设置M17、HOG、M17分化组（M17dif）、HOG分化组（HOGdif）四组，每组均设置3个生物学重复；每个样本均构建A-tailing RNaseR处理组与未处理组两种测序文库。