Analysis of transcrptomes of Danon patient derived hiPSC-cardiomyocytes by RNA-deep Sequencing

Control and Danon patients derived hiPSCs were differentiated into cardiomyocytes. RNA-seq was performed to analyze the gene expression differences between control and Danon lines. Overall design: hiPSC-CMs with over 90% purity were cultured in RPMI1640/B27 medium for about 50 days. Total RNAs were prepared from different hiPSC-CM lines and sequenced on a HiSeq 4000 sequencing system (Illumina).

本研究将来源于健康对照个体与Danon综合征患者的诱导多能干细胞（hiPSCs）定向分化为心肌细胞。采用RNA测序（RNA-seq）技术分析健康对照与Danon综合征患者来源细胞系之间的基因表达差异。实验整体设计：将纯度超过90%的诱导多能干细胞源性心肌细胞（hiPSC-CMs）于RPMI1640/B27培养基中培养约50天。随后从不同hiPSC-CM细胞系中提取总RNA，并在Illumina HiSeq 4000测序系统上完成测序。