Supporting data for thesis "p19Arf-Based Senolytic Vaccine Improves Age-Associated Pathologies and Extends Healthspan in Aging Mouse Models"

The dataset includes all raw data from the results section of the thesis, as well as processed data and images. The dataset has been divided into 7 subfolders based on chapters. Within these subfolders, the data is further organized into multiple folders by figure, with most folders containing data for a single figure and a few containing data for multiple figures. To locate the data for a specific figure, simply find the corresponding folder. Most of the data has been processed using Excel, and statistical graphs were generated using GraphPad software. The detailed descriptions for each folder are shown as followed:​<b>​Supporting Data for Chapter 3.1​</b>​<br>The dataset provides molecular validation of p19Arf as a senescence-associated target through qPCR and Western blot analyses, demonstrating its upregulated expression in senescent cells. Complementary senescence-associated β-galactosidase staining further confirmed the role of p19Arf in promoting cellular senescence, establishing its relevance in aging biology.​<b>​Supporting Data for Chapter 3.2​</b>​<br>This dataset encompasses the design and preclinical evaluation of p19Arf-derived peptide vaccines, where ELISPOT assays were employed to screen immunogenic candidates. Comprehensive safety assessments, including histological examinations of major organs, verified the absence of adverse effects in vaccinated mice, supporting the vaccine's biocompatibility. The folder includes high-resolution histopathology images for detailed review.​<b>​Supporting Data for Chapter 3.3​</b>​<br>In vitro studies utilized induced senescence models to investigate the vaccine's mechanism, with lactate dehydrogenase release assays confirming that T cells from immunized mice selectively targeted senescent cells. These results validate the vaccine's capacity to mediate specific clearance of senescent cells while preserving healthy populations.​<b>​Supporting Data for Chapter 3.4​</b>​<br>Experiments in accelerated aging mouse models evaluated the vaccine's therapeutic potential, with longitudinal monitoring of physiological and molecular aging markers. Observations included mitigated age-related weight fluctuations, preserved cognitive function, and reduced oxidative stress, collectively indicating deceleration of aging phenotypes.​<b>​Supporting Data for Chapter 3.5​</b>​<br>Analyses in naturally aged mice assessed systemic aging parameters, revealing modulated expression of senescence-associated genes and inflammatory markers following vaccination. The data highlight the vaccine's ability to counteract age-associated molecular dysregulation in physiological aging contexts.​<b>​Supporting Data for Chapter 3.6​</b>​<br>Structural and functional assessments demonstrated improvements in musculoskeletal health, with microCT imaging revealing enhanced bone architecture and treadmill tests showing prolonged physical endurance. These findings underscore the vaccine's benefits in maintaining mobility and skeletal integrity during aging.​<b>​Supporting Data for Chapter 3.7​</b>​<br>Long-term efficacy studies confirmed sustained immune memory responses and significant lifespan extension in vaccinated cohorts. Persistent antigen-specific T cell activity and delayed mortality onset were observed, supporting the vaccine's durability and potential as an intervention for healthy aging.<br>

本数据集涵盖本论文结果章节的全部原始数据、处理后数据及图像文件。本数据集按章节划分为7个子文件夹；各子文件夹内进一步以图表为单位组织为多个子目录，其中多数子目录对应单张图表的数据，少数子目录包含多张图表的相关数据。若需检索特定图表的数据，只需定位至对应子目录即可。本数据集多数数据通过Excel进行处理，统计图表则由GraphPad软件生成。各子目录的详细说明如下：<br><b>第3.1章配套支持数据</b><br>本数据集通过定量聚合酶链式反应（quantitative PCR，qPCR）与蛋白质免疫印迹（Western blot）分析，完成了p19Arf作为衰老相关靶点的分子验证，证实其在衰老细胞中表达上调。辅以衰老相关β-半乳糖苷酶染色实验，进一步确认了p19Arf在促进细胞衰老中的作用，确立了其在衰老生物学研究中的相关性。<br><b>第3.2章配套支持数据</b><br>本数据集包含p19Arf来源肽类疫苗的设计与临床前评价内容，通过酶联免疫斑点试验（enzyme-linked immunospot assay，ELISPOT）筛选具有免疫原性的候选疫苗。针对主要器官的组织学检查等全面安全性评估结果证实，接种疫苗的小鼠未出现不良反应，验证了该疫苗的生物相容性。本目录包含高分辨率组织病理学图像以供详细审阅。<br><b>第3.3章配套支持数据</b><br>本研究通过体外诱导衰老模型探究该疫苗的作用机制，乳酸脱氢酶释放实验证实，免疫小鼠的T细胞可选择性靶向并清除衰老细胞。上述结果验证了该疫苗能够介导衰老细胞的特异性清除，同时保留健康细胞群体。<br><b>第3.4章配套支持数据</b><br>本研究在加速衰老小鼠模型中评估该疫苗的治疗潜力，通过纵向监测生理与分子衰老标志物，观察到疫苗可缓解衰老相关的体重波动、维持认知功能并降低氧化应激水平，整体表明该疫苗可延缓衰老表型的进展。<br><b>第3.5章配套支持数据</b><br>本研究通过自然衰老小鼠分析系统衰老相关参数，结果显示接种疫苗后，衰老相关基因与炎症标志物的表达得到调控。上述数据凸显了该疫苗在生理性衰老过程中，对抗衰老相关分子失调的能力。<br><b>第3.6章配套支持数据</b><br>结构与功能评估结果证实该疫苗可改善肌肉骨骼健康：显微CT（microCT）成像显示骨结构得到增强，跑步机实验显示小鼠的运动耐力显著提升。上述发现强调了该疫苗在衰老过程中对运动能力与骨骼完整性的维持作用。<br><b>第3.7章配套支持数据</b><br>长期疗效研究证实，接种疫苗的小鼠群体可维持持久的免疫记忆应答，且寿命显著延长。实验观察到持续性的抗原特异性T细胞活性与延迟的死亡率，进一步支持该疫苗的长效性，以及其作为健康衰老干预手段的潜力。