Activation of a Hippocampal CREB-pCREB-miRNA-MEF2 Axis Modulates Individual Variation of Spatial Learning and Memory Capability

To identify novel miRNAs involved in the regulation of learning and memory formation, we used the Morris water maze task to distinguish inbred wild-type C57BL/6J mice with good or poor learning and memory capability. C57BL/6J mice which undergo water maze task were sacrificed immediately after training. The hippocampus was harvested for miRNAs expression analysis with miRNA array (Phalanx Mouse & Rat miRNA OneArray® 2.0, annotation based on: miRBase release 15). These differentially expressed miRNAs were applied for further investigation. The hippocampus in experiment mice underwent Morris water maze with good performances or poor performances were harvested immediately after training (n=4 in each groups, total 2(Replicates)*2(groups)=4 arrays were used).

为鉴定参与学习与记忆形成调控的新型微小RNA（miRNAs），本研究采用莫里斯水迷宫实验（Morris water maze task）对近交系野生型C57BL/6J小鼠的学习记忆能力进行分级，区分出学习记忆能力优异与欠佳的个体。接受水迷宫实验的小鼠于训练结束后即刻被处死，采集其海马组织进行miRNA表达分析，检测采用miRNA芯片（miRNA array），具体为Phalanx小鼠与大鼠miRNA OneArray® 2.0，注释信息基于miRBase第15版。所得差异表达miRNAs将用于后续研究。本实验中，经莫里斯水迷宫实验表现优异与表现不佳的小鼠海马组织均于训练后即刻采集，每组样本量为4，总计使用2（重复组）×2（组别）=4张芯片。