Transcriptomic Comparison of Common Nontyphoidal Salmonella Serovars Provides Greater Resolution for Understanding Differences in Virulence

RNA extracted from representative isolates of each serovar grown to late exponential phase in Luria-Bertani broth showed that transcript abundances of core genes were significantly higher (p < 0.05) than those of accessory genes for all three serovars. Inter-serovar comparisons demonstrated higher transcript abundances for genes related to vitamin B12 biosynthesis, and ethanolamine and 1,2-propanediol utilization in S. Cerro compared to Javiana and Typhimurium, while genes in Salmonella Pathogenicity Island (SPI) 1 had significantly higher transcript abundances in S. Javiana and S. Typhimurium. mRNA profiles from three Salmonella serovars (Cerro, Javiana, Typhimurium) were generated by deep sequencing with an Illumina NextSeq 500 in triplicate with 75-nt stranded single-end reads.

从在卢里亚-贝塔尼肉汤（Luria-Bertani broth，LB肉汤）中培养至指数生长后期的各血清型（serovar）代表分离株中提取的RNA检测结果显示，三种血清型的核心基因（core genes）转录本丰度均显著高于附属基因（accessory genes）（P < 0.05）。血清型间比较分析表明，相较于贾维安娜沙门氏菌（S. Javiana）与鼠伤寒沙门氏菌（S. Typhimurium），塞罗沙门氏菌（S. Cerro）中与维生素B12生物合成、乙醇胺及1,2-丙二醇利用相关的基因转录本丰度更高；而沙门氏菌致病岛1（Salmonella Pathogenicity Island，SPI1）内的基因在贾维安娜沙门氏菌和鼠伤寒沙门氏菌中的转录本丰度显著更高。本数据集通过深度测序获得了三种沙门氏菌血清型（塞罗、贾维安娜、鼠伤寒）的mRNA表达谱，采用伊卢米纳NextSeq 500（Illumina NextSeq 500）测序平台，所有实验均设置三次生物学重复，使用75个核苷酸的链特异性单端测序读段。