Identification of target genes of Arabidopsis NIGT1 subfamily members (AtNIGT1s). Arabidopsis thaliana

Nitrogen (N) is a key nutrient that is often the limiting factor in plant growth. However, the molecular mechanisms underlying transcriptional regulation of N-starvation-responses remain largely unknown. AtNIGT1s are putative regulators of nitrogen-starvation responsive transcriptome in arabidopsis. We aimed to identify AtNIGT1s target genes by microarray experments. Overall design: AtNIGT1.2 overexpressor and AtNIGT1.2-VP overepxressor were subjected to microarray analysis to identify Direct Target genes.

氮（Nitrogen，N）是一类关键营养元素，通常为限制植物生长的关键因子。然而，氮饥饿响应的转录调控分子机制目前仍未被充分阐明。AtNIGT1家族是拟南芥中氮饥饿响应转录组的推定调控因子。本研究旨在通过芯片实验（microarray）鉴定AtNIGT1的靶基因。实验整体设计：将AtNIGT1.2过表达株系与AtNIGT1.2-VP过表达株系进行芯片分析，以鉴定其直接靶基因。