Adverse early-life experiences are associated with stress-response behavioral alteration, hypothalamic-pituitary-adrenal axis hyperactivity, and a larger pituitary gland volume in adult CD-1 male mice

Mice's behavior was evaluated by forced swim test. Tissues were analyzed by ELISA assays. Analysis of brain and pituitary was performed using anatomic magnetic resonance imaging, and the diffusion-weighted imaging techniques (diffusion-weighted imaging and apparent diffusion coefficient).

本研究通过强迫游泳实验（forced swim test）评估小鼠行为学表现；采用酶联免疫吸附试验（ELISA）分析组织样本；利用解剖磁共振成像（anatomic magnetic resonance imaging）及弥散加权成像技术（涵盖弥散加权成像（diffusion-weighted imaging）与表观扩散系数（apparent diffusion coefficient））对大脑及垂体组织开展检测分析。