Data for System Analysis of Lipomyces starkeyi During Growth on Various Plant‑Based Sugars

Oleaginous yeasts have received significant attention due to their substantial lipid storage capability. The accumulated lipids can be utilized directly or processed into various bioproducts and biofuels. Lipomyces starkeyi is an oleaginous yeast capable of using multiple plant-based sugars, such as glucose, xylose, and cellobiose. It is, however, a relatively unexplored yeast due to limited knowledge about its physiology. In this study, we have evaluated the growth of L. starkeyi on different sugars and performed transcriptomic and metabolomic analyses to understand the underlying mechanisms of sugar metabolism. Principal component analysis showed clear differences resulting from growth on different sugars. We have further reported various metabolic pathways activated during growth on these sugars. We also observed non-specific regulation in L. starkeyi and have updated the gene annotations for the NRRL Y-11557 strain. This analysis provides a foundation for understanding the metabolism of these plant-based sugars and potentially valuable information to guide the metabolic engineering of L. starkeyi to produce bioproducts and biofuels.

产油酵母（oleaginous yeast）凭借其卓越的脂质储存能力受到广泛关注。其胞内积累的脂质可直接利用，或加工为各类生物制品与生物燃料。斯达氏油脂酵母（Lipomyces starkeyi）是一类可利用葡萄糖、木糖、纤维二糖等多种植物源糖类的产油酵母，但由于对其生理特性的认知较为有限，目前相关研究程度相对较浅。本研究针对斯达氏油脂酵母在不同糖类底物上的生长情况进行了评估，并开展转录组学与代谢组学分析，以解析其糖类代谢的潜在分子机制。主成分分析（Principal Component Analysis, PCA）结果显示，以不同糖类为底物培养的菌体生长状态存在显著差异。本研究进一步阐明了该菌株在上述糖类上生长时激活的各类代谢通路，同时观察到达氏油脂酵母存在非特异性调控现象，并对NRRL Y-11557菌株的基因注释进行了更新。本研究为解析该菌株对植物源糖类的代谢机制提供了理论基础，同时也为通过代谢工程改造斯达氏油脂酵母以生产生物制品与生物燃料提供了具有参考价值的信息。