Postzygotic barriers persist despite ongoing introgression in hybridizing Mimulus species

Using inbred lines of Mimulus guttatus and M. nasutus derived from the sympatric site Catherine Creek (CAC), we produced three Recombinant Inbred Line (RIL) populations from crosses between a common M. nasutus maternal parent CAC9 and the M. guttatus paternal parents CAC110, CAC162, and CAC415. We generated RADseq data from these RILs. Two rounds of RIL libraries were prepared and sequenced due to insufficient sequencing coverage from the first round, therefore, multiple fastq files are associated with each RIL individual. We characterize transmission ratio distortion, long distance linkage disequilibrium, and hybrid incompatibilities in these populations.

本研究以采自同域分布位点凯瑟琳溪（Catherine Creek, CAC）的沟酸浆（Mimulus guttatus）与那氏沟酸浆（Mimulus nasutus）近交系为实验材料，以通用的那氏沟酸浆母本CAC9作为共同亲本，分别与沟酸浆父本CAC110、CAC162、CAC415杂交，构建了3个重组自交系（Recombinant Inbred Line, RIL）群体。
我们对上述RIL群体开展了限制性位点关联DNA测序（RADseq）。由于首轮测序的覆盖度不足，共完成两轮RIL文库的构建与测序工作，因此每个RIL个体对应多个FASTQ文件。
本研究对这些群体中的传递比率偏倚、远距离连锁不平衡以及杂交不亲和性进行了表征分析。