Expression Data from the Riesling grape must fermentation by industrial M2 and M2 nsf1∆ S. cerevisiae strains for functional characterization of the NSF1 (YPL230W) gene via Correlation Clustering-based method

The main objectives of this study were to expand our understanding of NSF1 gene function in industrial S. cerevisiae M2 strain during fermentation by finding the largest maximal clique of co-expressed genes (i.e. Interdependent Correlation Cluster), and to establish the impact of Nsf1p on genome-wide gene expression during the fermentation process with possible implications related to wine quality and S. cerevisiae adapation to stressful fermentation conditions The Affymetrix Yeast 2.0 microarrays were used to capture the global gene expression profile of M2 and M2 nsf1∆ grown under fermentation conditions in Riesling grape must at 18°C with no shaking at various time points. The analysis of this microarray dataset expanded our understanding of the mechanism of action and the roles of NSF1 under fermentation stress conditions. The overall experimental setup consisted of 2 stains (M2 and M2 nsf1∆) and 3 sample time points (24h post-innoculation, 20% and 85% of total glucose fermented) .

本研究的核心目标分为两点：其一，通过筛选协同表达基因的最大极大团（即互依赖相关簇，Interdependent Correlation Cluster），加深对工业酿酒酵母（Saccharomyces cerevisiae，简称S. cerevisiae）M2菌株中NSF1基因（NSF1 gene）在发酵过程中的功能认知；其二，明确Nsf1蛋白（Nsf1p）对发酵过程中全基因组基因表达的调控影响，相关发现可为葡萄酒品质优化以及酿酒酵母适应发酵胁迫环境的研究提供理论参考。本研究采用Affymetrix酵母2.0基因芯片（Affymetrix Yeast 2.0 microarrays），在18℃、静置培养条件下以雷司令葡萄汁为发酵基质培养M2野生型菌株与M2 nsf1Δ基因敲除菌株，并分别于接种后24h、总葡萄糖发酵度达20%和85%三个时间点采集样本，获取二者的全基因组表达谱。对该基因芯片数据集的分析，进一步拓展了我们对NSF1基因在发酵胁迫环境下的作用机制与生物学功能的理解。本实验的整体设置包含2组菌株（M2与M2 nsf1Δ）以及3个样本采集时间点：接种后24h、总葡萄糖发酵度达20%以及总葡萄糖发酵度达85%。