Supporting data for "The role and mechanisms of interleukin-9 in facilitating anticancer effect of immune checkpoint blockade in lung cancer animal model".

This dataset includes the data used in thesis entitled "The role and mechanisms of interleukin-9 in facilitating anticancer effect of immune checkpoint blockade in lung cancer animal model". Laboratory research data includes: 1) Western blot: Images will be scanned and saved as .jpg or .tif files. Measurements and quantification of images can be recorded into excel spreadsheets. 2) MTT: MTT datasets are exported as .xlsx excel files, showing the OD values at 570nm and the test date. 3) Tumor volume will be recorded every other day as three diameters in a .xlsl file, documenting the treatment time and groups. 4) Flow cytometry: Raw flow cytometry files saved from Cotyflex will be in the format of .fcs. Fowjo is used to analyze the .fcs files. Final datasets will be saved as a .wsp file and images will be around 500MB. 5) RT-qPCR: Real-time exponential graphs and data generated by the StepOne program will be exported as .csv excel files and .eds raw data files. Files generated throughout the entire course will be approximately 1-1.5 GB. 6) Statistical analysis software includes GraphPad Prism and ImageJ, files will be saved in their respective intrinsic/proprietary formats.本数据集涵盖题为《白细胞介素9 (interleukin-9)在肺癌动物模型中促进免疫检查点阻断(immune checkpoint blockade)抗肿瘤作用的角色与机制》的学位论文所使用的全部实验数据。实验室研究数据具体如下： 1) 蛋白质印迹（Western blot）：实验图像将被扫描并保存为.jpg或.tif格式文件，对图像的定量与测量结果可记录至Excel电子表格中。 2) MTT实验：MTT数据集以.xlsx格式的Excel文件导出，其中包含570nm处的光密度（OD）值与实验日期。 3) 肿瘤体积：每两日记录一次，包含三条径线数据，相关信息存储于.xlsx文件中，同时记录处理时间与实验组别。 4) 流式细胞术：由Cotyflex设备导出的原始流式细胞术文件保存为.fcs格式，使用FlowJo对.fcs文件进行分析处理，最终数据集保存为.wsp格式文件，配套图像总大小约为500MB。 5) 实时定量聚合酶链反应（RT-qPCR）：由StepOne程序生成的实时扩增曲线与相关数据将分别导出为.csv格式Excel文件与.eds原始数据文件。整个实验周期内生成的全部文件总容量约为1-1.5 GB。 6) 本研究使用的统计分析软件包括GraphPad Prism与ImageJ，相关结果文件将以各自专属的原生专有格式进行保存。