Genomic DNA from dCas9-mediated precipitation of telomere chromatin in plant leaves

We used a biotinylated dCas9 to specifically target and precipitate the telomeric fraction of fragmented chromatin in V. vinifera cv Thompson Seedless and Cabernet Franc, as well as S. lycopersicum leaf nuclei.

本研究使用生物素标记的失活Cas9（biotinylated dCas9），特异性靶向并沉淀葡萄（Vitis vinifera）栽培品种汤普森无籽与品丽珠的破碎染色质中端粒组分，以及番茄（Solanum lycopersicum）叶片细胞核内的端粒组分。