Table_1_Effects of high oleic acid peanuts on mice’s liver and adipose tissue metabolic parameters and gut microbiota composition.docx

This study aimed to investigate the effects of two types of peanuts, regular Hanoch (HN) and a new high-oleic cultivar., Hanoch-Oleic (HO), on metabolic parameters and gut microbiota composition. Male C57BL/6 mice were fed with a normal diet (ND) or ND supplemented with HN (NDh) or HO (NDo). Following 18 weeks of diet regimen, the NDo group exhibited reduced body weight and peri-gonadal adipose-to-body weight ratio, paralleled to lesser food consumption. Although blood levels of total cholesterol, HDL-cholesterol, free fatty acids, and liver enzyme levels did not differ between groups, decreased insulin sensitivity was found in the NDh group. Within adipose tissue, the expression of lipolytic and lipogenic enzymes was higher, while those related to lipid oxidation were lower in the NDh group compared to the NDo group. Additionally, HO peanuts consumption promoted the establishment of a healthy microbiota, with an enhanced abundance of Bifidobacterium, Lactobacillus, and Coprococcus genera. In conclusion, the inclusion of the HO peanut cultivar., rather than the conventional peanut cultivar., in a balanced diet was related to better metabolic outcomes and was linked to a favorable microbiota profile.

本研究旨在探究两种花生品种——普通哈诺奇（Hanoch, HN）与新型高油酸品种哈诺奇油酸（Hanoch-Oleic, HO）——对代谢指标及肠道菌群（gut microbiota）组成的影响。将雄性C57BL/6小鼠分为三组，分别饲喂基础日粮（normal diet, ND）、基础日粮添加HN（NDh）以及基础日粮添加HO（NDo）。经过18周的饲喂周期后，NDo组小鼠的体重及性腺周脂肪/体重比值均显著降低，且进食量更少。尽管各组小鼠的血清总胆固醇、高密度脂蛋白胆固醇（HDL-cholesterol）、游离脂肪酸水平及肝酶活性均无显著差异，但NDh组小鼠的胰岛素敏感性出现下降。与NDo组相比，NDh组小鼠脂肪组织中脂解酶与生脂酶的表达水平更高，而与脂质氧化相关酶的表达水平更低。此外，饲喂HO花生可促进健康肠道菌群的建立，使双歧杆菌属（Bifidobacterium）、乳杆菌属（Lactobacillus）及柯林斯菌属（Coprococcus）的丰度显著提升。综上，在均衡日粮中添加HO花生品种而非普通花生品种，可带来更优的代谢结局，并与更健康的肠道菌群特征相关。