Passive leakage across monolayers using calcein as a fluorescent marker

This dataset tabulates data on passive leakage across the monolayers using a fluorescent marker, calcein, a derivative of fluorescein. The purpose was to gauge the degree of confluence and whether tight junctions had formed between the epithelial cells.
The sheet 'Leak rates' tabulates a summary of the data of the 17 calcein experiments in the raw data sheet. The sheet 'Raw data' contains the data and calculations of the 17 experiments.

本数据集详尽记录了使用荧光标记物——钙黄绿素（calcein），一种荧光素衍生物——在单层膜中进行的被动泄漏数据。其旨在评估细胞聚集程度以及上皮细胞之间是否形成了紧密连接。'泄漏率'工作表汇总了原始数据表中17次钙黄绿素实验的数据概览。'原始数据'工作表则包含了17次实验的数据及计算过程。