HDR-CRISPR promotes targeted genome editing with reduced mutational burden in primary human cells [CAST-seq]

We have established a technology to endorse DSB resolution via HDR with the goal of facilitating clinical transition of HDR-based editing strategies and increase safety. We have used high throughput sequencing as deposited here to assess safety of our platform Overall design: We have performed CAST-seq to monitor gross genomic aberration between control samples and samples that have received our HDR-promoting tools

我们开发了一项可通过同源定向修复（Homology-Directed Repair, HDR）介导DNA双链断裂（Double-Strand Break, DSB）修复的技术，旨在推动基于HDR的基因编辑策略向临床转化，并提升编辑安全性。我们采用本文所存档的高通量测序技术，对本研发平台的安全性进行评估。整体实验设计：我们通过CAST-seq技术，对对照组样本与接受本研究开发的HDR促进工具的样本之间的全基因组大范围染色体畸变情况进行监测。