Assessing the effects of adsorptive polymeric resin additions on fungal secondary metabolite chemical diversity

Adsorptive polymeric resins have been occasionally described to enhance the production of specific secondary metabolites (SMs) of interest. Methods that induce the expression of new chemical entities in fungal fermentations may lead to the discovery of new bioactive molecules and should be addressed as possible tools for the creation of new microbial chemical libraries for drug lead discovery. Herein, we apply both biological activity and chemical evaluations to assess the use of adsorptive resins as tools for the differential expression of SMs in fungal strain sets. Data automation approaches were applied to ultra high performance liquid chromatography analysis of extracts to evaluate the general influence in generating new chemical entities or in changing the production of specific SMs by fungi grown in the presence of resins and different base media.吸附性聚合物树脂（adsorptive polymeric resins）偶有报道可提升具有研究价值的特定次级代谢产物（secondary metabolites, SMs）的合成产量。可诱导真菌发酵过程中新型化学实体表达的方法，有助于发现全新生物活性分子，应作为构建新型微生物化学库以用于药物先导物发现的潜在工具加以研究。本文中，我们同时通过生物学活性与化学评价手段，评估吸附性聚合物树脂作为工具，在真菌菌株集内差异化表达次级代谢产物的应用效果。我们采用数据自动化方法对提取物的超高效液相色谱分析结果进行处理，以综合评估在树脂与不同基础培养基存在下培养的真菌，其生成新型化学实体或改变特定次级代谢产物产量的整体影响。