SHANK2A and SHANK2A(R841X) mouse brain SWATH quantitative proteomics

The high molecular complexity of the SHANK scaffold and its fundamental role in neural circuitries governing social behavior is not well understood. Here we analyzed two newly generated transgenic mouse lines with the SHANK2A wild-type isoform and the other with SHANK2A carrying a SHANK2A(R841X) point mutation. The proteome of hippocampal synapse-enriched fraction for both lines was investigated by quantitative SWATH proteomic analysis in order to identify synaptic proteins and signaling pathways that respond to the SHANK2 isoforms.

SHANK支架蛋白（SHANK scaffold）的高分子复杂性，及其在调控社会行为的神经环路中发挥的核心作用，目前尚未得到充分阐明。本研究分析了两株新构建的转基因小鼠品系：其一表达SHANK2A野生型亚型，另一株则携带带有SHANK2A(R841X)点突变的SHANK2A亚型。针对这两株小鼠，我们通过定量SWATH蛋白质组学分析对其海马突触富集组分的蛋白质组开展了研究，旨在鉴定出响应SHANK2亚型的突触蛋白与信号通路。