Table_1_Identification of complex III, NQR, and SDH as primary bioenergetic enzymes during the stationary phase of Pseudomonas aeruginosa cultured in urine-like conditions.xlsx

Pseudomonas aeruginosa is a common cause of urinary tract infections by strains that are often multidrug resistant, representing a major challenge to the world’s health care system. This microorganism has a highly adaptable metabolism that allows it to colonize many environments, including the urinary tract. In this work, we have characterized the metabolic strategies used by stationary phase P. aeruginosa cells cultivated in urine-like media to understand the adaptations used by this microorganism to survive and produce disease. Our proteomics results show that cells rely on the Entner-Duodoroff pathway, pentose phosphate pathway, the Krebs cycle/ glyoxylate shunt and the aerobic oxidative phosphorylation to survive in urine-like media and other conditions. A deep characterization of the oxidative phosphorylation showed that the respiratory rate of stationary phase cells is increased 3–4 times compared to cells in the logarithmic phase of growth, indicating that the aerobic metabolism plays critical roles in the stationary phase of cells grown in urine like media. Moreover, the data show that respiratory complex III, succinate dehydrogenase and the NADH dehydrogenase NQR have important functions and could be used as targets to develop new antibiotics against this bacterium.

铜绿假单胞菌（Pseudomonas aeruginosa）是多耐药菌株引发尿路感染的常见致病菌，对全球医疗体系构成重大挑战。该微生物拥有高度适应性的代谢系统，使其能够定植于包括泌尿道在内的多种环境中。本研究对在类尿培养基中培养的铜绿假单胞菌稳定期细胞所采用的代谢策略进行了表征，以阐明该微生物用于存活与致病的适应机制。本研究的蛋白质组学结果显示，细胞在类尿培养基及其他环境中存活时，依赖肠-杜道夫途径（Entner-Duodoroff pathway）、磷酸戊糖途径、三羧酸循环/乙醛酸旁路以及有氧氧化磷酸化通路。对有氧氧化磷酸化的深入解析表明，稳定期细胞的呼吸速率较对数生长期细胞提升3~4倍，这提示有氧代谢在类尿培养基中培养的细胞稳定期发挥关键作用。此外，研究数据显示，呼吸复合物III、琥珀酸脱氢酶以及NADH脱氢酶NQR发挥着重要功能，可作为开发针对该致病菌的新型抗生素的靶点。