Manual nuclei segmentations in near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of Interphase COS-7 cell (jrc_cos7-1b)

Annotation description: Dense segmentations of nuclei in jrc_cos7-1b using Amira-Avizo (Thermo Fisher Scientific) and "Using Amira to manually segment organelles in vEM for machine learning V.3" protocol.Annotation protocol: Using Amira to manually segment organelles in vEM for machine learning V.3 (http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3)Software: Amira 3D 2021.1, Classic Segmentation WorkroomClasses annotated: NucleiSource (EM) dataset ID: jrc_cos7-1bSource (EM) voxel size (nm): 2 x 2 x 2 (X, Y, Z)Source (EM) data dimensions (µm): 40.69 x 9.05 x 2.23 (x, y, z)Source (EM) DOI: https://doi.org/10.25378/janelia.26512297Dataset URL: s3://janelia-cosem-datasets/jrc_cos7-1b/jrc_cos7-1b.zarr/recon-2/labels/inference/segmentations/nuc/Visualization website: https://openorganelle.janelia.org/datasets/jrc_cos7-1b标注说明：使用Amira-Avizo（Thermo Fisher Scientific，赛默飞世尔科技）及《用于机器学习的体视电子显微镜（volume EM，vEM）中细胞器手动分割（V.3版）》（Using Amira to manually segment organelles in vEM for machine learning V.3）协议，对jrc_cos7-1b数据集内的细胞核进行密集型分割标注。 标注协议：《用于机器学习的体视电子显微镜（volume EM，vEM）中细胞器手动分割（V.3版）》（Using Amira to manually segment organelles in vEM for machine learning V.3，http://dx.doi.org/10.17504/protocols.io.bp2l61rb5vqe/v3） 所用软件：Amira 3D 2021.1、经典分割工作间（Classic Segmentation Workroom） 标注类别：细胞核（Nuclei） 电子显微镜（Electron Microscopy, EM）数据集编号：jrc_cos7-1b 电子显微镜（Electron Microscopy, EM）体素尺寸（单位：纳米）：2×2×2（X、Y、Z轴） 电子显微镜（Electron Microscopy, EM）数据尺寸（单位：微米）：40.69×9.05×2.23（x、y、z轴） 电子显微镜（Electron Microscopy, EM）数据集DOI：https://doi.org/10.25378/janelia.26512297 数据集存储地址：s3://janelia-cosem-datasets/jrc_cos7-1b/jrc_cos7-1b.zarr/recon-2/labels/inference/segmentations/nuc/ 可视化网站：https://openorganelle.janelia.org/datasets/jrc_cos7-1b